Abstract: The Jiaochong Au-S ore deposit is located in the Tongling ore concentration area along the middle-lower reaches of the Yangtze River. The ore body mainly occurs in the Lower Permian Qixia Formation. Based on an analysis of geological and ore-forming conditions, this paper systematically focuses on the composition characteristics of S, Pb isotopes in the sulfide ore minerals, and also discusses the sources of the ore-forming materials. Studies have shown that the composition of S isotope in sulfide ore minerals has a narrow variation range, and the δ34SΣS in the ore-forming hydrothermal system is about 4.0‰. It is inferred that the S was genetically closely related to the magmatic sulfur. The isotopic compositions of ore lead vary greatly and belong to anomalous lead. Finally, through the chart of Zartman`s Pb evolutionary tectionic settings and Δγ-Δβ diagram of genetic classification, the authors consider that the ore-forming materials of the ore deposit were closely related to the magmatism and derived from the mixed sources of the upper continental crust and the mantle. The authors sincerely hope that these data can provide some help for the study of ore deposits related to granodiorite of the Tongling ore concenteation area.