Abstract: With the development of urban agglomeration in China, the integrated development of urban agglomeration has become a national strategy.However, cross-regional engineering construction is faced with the problems of disunity of engineering geological stratum division standards and unsystematic engineering geological zoning.The regional sharing of engineering geological data and the integrated development of major projects are restricted.Based on the evolution of sedimentary environment of Quaternary soils, an engineering geological sequence determination method is present in this paper, which integrates the structural characteristics, physical-mechanical conditions and spatial distribution characteristics of soils.On this basis, the key geological strata affecting the engineering construction are identified, and the engineering geological zoning method based on the distribution of key engineering geological strata is proposed.Finally, taking the area along the Yangtze River in Anhui Province as an example, the geomorphic characteristics and Quaternary stratigraphic characteristics are systematically analyzed through engineering geological survey of thirtythree 1:50000 standard maps.Standard engineering geological strata have been established, including 5 main layers and 17 sub-layers.Besides, engineering geological zoning has been established, including 2 first-level zones and 6 sub-zones.The results provide an engineering geological foundation framework for engineering planning and construction of along the Yangtze River in Anhui Province.The research results can provide new ideas for stratigraphic ordering and regionalization of engineering geology of urban agglomeration in river valley plain.