Abstract: MT and AMT profiling was conducted in Mangniuhai sag area of Tuquan basin so as to evaluate the potential of oil and gas resources associated with the late Paleozoic and Mesozoic strata. The results of MT and AMT profile survey shows that there are two thick and transverse continuous distribution conductive layers under the volcanic layer at the depth of 800~2000m and 2000~8000m, respectively. By combining drilling material and regional geological material, the authors consider that the first conductive layer represents the coal strata of early Jurassic, and the second conductive layer represents the mudstone layer of upper Paleozoic. Based on the results of ground hydrocarbon source rocks survey, it can be predicted that both the conductive layers could contain excellent hydrocarbon source rocks, which means that there exists great potential for detecting the upper Paleozoic and the early Mesozoic hydrocarbon resources in Tuquan basin.