露采矿山恢复模式对土壤理化性质和微生物群落结构的影响

    Impacts of restoration mode on soil physicochemical properties and microbial community structure in an open-pit mining area

    • 摘要: 【研究目的】露天采矿严重破坏土壤结构和区域生态系统健康。露采矿山复绿过程中土壤是生态恢复的关键,厘清不同恢复模式对土壤理化性质和微生物群落结构的影响,对探索矿区生态恢复措施及成效评价极为重要。【研究方法】本研究以浙江杭州废弃露采矿区的三种不同恢复模式(陡坡裸地、人工恢复、自然恢复)下的土壤为研究对象,通过调查土壤理化性质、土壤酶活性和土壤微生物群落结构,探讨露采矿山复绿过程中不同恢复模式对土壤理化性质和土壤微生物群落结构的影响。【研究结果】结果表明,自然恢复和人工恢复下的土壤有机碳、全氮、水解性氮含量较裸地显著增加,但低于周边未开采区森林土壤;人工恢复下的土壤酸性磷酸酶、N-乙酰-β-D-葡萄糖苷酶、亮氨酸氨基肽酶和β-葡萄糖苷酶活性显著升高。土壤真菌和真菌的Chao1指数和Shannon均表现为自然恢复>人工恢复;未修复裸地的土壤真菌多样性为最低;细菌优势门为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和酸杆菌门(Acidobacteria)。真菌优势门为子囊菌门(Ascomycota)和担子菌门(Basidiomycota)。土壤有效磷、pH和速效钾是显著影响细菌群落结构的环境因子,土壤真菌群落结构与土壤全磷、有效磷、pH、含水量、全氮、水解性氮呈显著负相关。【结论】可见,人工恢复下土壤恢复较快,土壤理化性质、酶活性和微生物群落多样性表现出较好的恢复效果;自然恢复下土壤恢复缓慢,但利于土壤微生物群落结构和多样性的恢复。研究结果可为筛选矿山复绿过程中生态功能恢复的有效途径、优化矿山复绿策略提供科学依据。

       

      Abstract: Objective Open-pit mining has seriously damaged soil structure and regional ecosystem health. It is imperative to recognize the pivotal role of soil in the process of ecological restoration in the context of reforestation following open-pit mining. It has therefore become imperative to recognize the effects of different restoration modes on the physicochemical properties and microbial community structure of soil which is important for exploring the ecological restoration measures of mining areas and evaluating the effectiveness of these measures. Methods In this study, we investigated the effects of three restoration methods (bare mining area, artificial restoration, and natural restoration) on the soils of abandoned open-pit mining area in Hangzhou, Zhejiang Province. The impacts of different restoration methods on soil physicochemical properties and soil microbial community structure were investigated through the investigation of the soil physicochemical properties, the soil enzyme activities, and the structure of the soil microbial community. Results The results showed that the contents of soil organic carbon, total nitrogen, and hydrolyzable nitrogen under both natural and artificial restoration increased significantly compared to that of bare mining areas, but remained lower than that of surrounding forest soils. Furthermore, the activities of soil acid phosphatase, N-acetyl-β-D-glucosidase, leucine aminopeptidase, and β-glucosidase were found to be significantly higher under artificial restoration. The Chao1 index and Shannon diversity index of soil fungi and bacteria indicated that natural restoration method exhibited greater efficacy than artificial restoration, with the lowest diversity observed in bare mining areas. The dominant phyla of bacterial were Proteobacteria, Actinobacteria, and Acidobacteria, while the dominant fungal phyla were Ascomycota and Basidiomycota. Soil effective phosphorus, pH and quick-acting potassium were the environmental factors that significantly affected the bacterial community structure. In addition, the soil fungal community structure was significantly negatively correlated with soil total phosphorus, effective phosphorus, pH, water content, total nitrogen and hydrolyzable nitrogen. Conclusions The process of soil recovery under artificial restoration is accelerated, and the soil physicochemical properties, enzyme activity and microbial community diversity demonstrate a more pronounced recovery effect. Conversely, soil recovery under natural restoration is gradual, yet it is conducive to the recovery of soil microbial community structure and diversity. The findings of the study provide a scientific foundation for the identification of effective methods for ecological function recovery in the context of mining re-greening and the optimisation of mining re-greening strategies.

       

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