Abstract: Ecological zoning from the perspective of coupling the supply and demand of the ecosystem services is instrumental in promoting the sustainable development of social−ecological system. In this study, the ecosystem services supply (ESS) and ecosystem services demand (ESD) the between 2010 and 2020 in Chishui River Basin were investigated. The coupling degrees were calculated based on the changes in ESS and ESD between 2010 and 2020. According to the results of coupling degree, the 409 study units (streets/towns) were divided into four categories. The averaged ESS changes for the high order of coupling degrees zonation, high order of uncoupling degree zonation, low order of uncoupling degree zonation and low order of uncoupling degree zonation are 1.7 ± 2.1, −0.9 ± 2.2, 5.9 ± 6.4 and −0.1 ± 0.5, respectively. The averaged ESD changes are 0.6 ± 1.1, 2.1 ± 2.4, 0.7 ± 2.4 and −0.5 ± 0.4, respectively. The 61 streets/towns with high order of coupling degrees represented for conversation−oriented zone in which should focus on conservation on the key and typical landscapes, improve the existing ecosystem network and highlight the regional social−ecological complex functions. The 140 streets/towns with high order of uncoupling degree represented for key restoration zone, in which focused on incorporating key areas for ecological restoration into regional development planning. The 63 streets/towns with low order of uncoupling degree represented for network−oriented zone, focusing at establishing ecological corridors and networks to improve the connectivity, so as to maintain important the continuous supply of ecosystem services in the region. The 145 streets/towns with low order of uncoupling degree represented for ecological livelihood−oriented zone focusing at alternative livelihood through ecological branding and products value gaining. The finding of the ecological zoning scheme of the Chishui River Basin reflects the differences in the spatial allocation of regional environmental resources and can provide technical guidance on spatial planning for ecological conservation and restoration.